Review



pires2 egfp vector  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    TaKaRa pires2 egfp vector
    Pires2 Egfp Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 422 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pires2+egfp+vector/pIRES2-AcGFP1+Vector/bio_rxiv__2025__08__24__671966-144-12-14
    Average 96 stars, based on 422 article reviews
    pires2 egfp vector - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: Actin-based deformations of the nucleus control mouse multiciliated ependymal cell differentiation.
    Article Snippet: The pCMV HA hRB1 delta CDK63 was a gift from S. Dowdy (Addgene plasmid # 58906; http://n2t.net/addgene:58906; RRID:Addgene_58906). .. HA hRB1 delta CDK was subcloned at BamHI restriction site on pIRES2-EGFP vector (Clontech). .. The MCIDAS-GFP construct81 was a gift from S. Taraviras, in our hand the GFP was barely detectable. pCMV-tdTomato is from Clontech.

    Article Title: Optogenetic Control of Receptor-mediated Growth Cone Dynamics in Neurons
    Article Snippet: Received: Jul 26, 2023 Revised: Dec 3, 2024 Accepted: Dec 10, 2024 ABSTRACT Development of neuronal connections is spatially and temporally controlled by extracellular cues which often activate their cognate cell surface receptors and elicit localized cellular responses.. Here, we demonstrate the use of an optogenetic tool to activate receptor signaling locally to induce actin-mediated growth cone remodeling in neurons.. Based on the light-induced interaction between Cryptochrome 2 (CRY2) and CIB1, we generated a bicistronic vector to co-expresses CRY2 fused to the intracellular domain of a guidance receptor and a membrane-anchored CIB1.

    Article Title: A humanized monoclonal antibody targeting an ectonucleotidase rescues cardiac metabolism and heart function after myocardial infarction
    Article Snippet: .. MGC Sequence-Verified human cDNAs of ENPP4, ENPP5, CD39, CD73 (Horizon Discovery), and ENPP3 (Cat# RDC2698, R&D Systems) were cloned into the pIRES2-EGFP vector (Clontech) for expression studies. ..

    Article Title: Puromycin-sensitive aminopeptidase acts as an inhibitory auxiliary subunit of volume-regulated anion channels
    Article Snippet: Myc-Flag-tagged cDNAs of human SWELL1 (NM_019594), LRRC8B (NM_015350), LRRC8C (NM_032270), LRRC8E (NM_025061) in pCMV vector were obtained from OriGene (RC208632, RC205553, RC222603, RC209849). .. The coding sequences without a tag were PCR amplified and sub-cloned into pIRES2-EGFP vector (Clontech). .. The cDNA of human LRRC8D (NM_018130) in pCMV6-Entry vector was obtained from Amsbio(SC319580).

    Article Title: A humanized monoclonal antibody targeting an ectonucleotidase rescues cardiac metabolism and heart function after myocardial infarction.
    Article Snippet: MGC Sequence-Verified human cDNAs of ENPP4, ENPP5, CD39, CD73 (Horizon Discovery), and ENPP3 (Cat# RDC2698, R&D Systems) were cloned into the pIRES2-EGFP vector (Clontech) for expression studies. .. MGC Sequence-Verified human cDNAs of ENPP4, ENPP5, CD39, CD73 (Horizon Discovery), and ENPP3 (Cat# RDC2698, R&D Systems) were cloned into the pIRES2-EGFP vector (Clontech) for expression studies. ..

    Article Title: Enforced MYC expression directs a distinct transcriptional state during plasma cell differentiation
    Article Snippet: .. The MYC TAD MB deletion mutants, as well as the mutation of MYC 132-135 amino acid sequence, DCMW, to alanine residues, and the single substitution W135A were commercially synthesized with the t2A-BCL2 sequence included in the insert and cloned into the pIRES2-EGFP vector (Clontech). ..

    Article Title: Enforced MYC expression directs a distinct transcriptional state during plasma cell differentiation.
    Article Snippet: .. The MYC TAD MB deletion mutants, as well as the mutation of MYC 132-135 amino acid sequence, DCMW, to alanine residues, and the single substitution W135A were commercially synthesized with the t2A-BCL2 sequence included in the insert and cloned into the pIRES2-EGFP vector (Clontech). ..

    Construct:

    Article Title: Melatonin Enhances Sleep via MT 1 -Driven Activation of Slo1 in Suprachiasmatic Nucleus Neurons
    Article Snippet: Normalized values were exported to Origin software (OriginLab, USA) for calculating the mean ± standard error (SE) and generating graphical representations. .. The plasmids encoding hemagglutinin (HA)-tagged mouse MT 1 ( wp2117 ) and FLAG-tagged mouse Slo1 ( wp2116 ) were constructed by cloning PCR-amplified products into the pIRES2–EGFP vector (Clontech, Mountain View, CA, USA). .. The Mtnr1a cDNA (NM_008639, MR224599, OriGene, Rockville, MD, USA) and Slo1 cDNA (MBr5/3, provided by Dr. Lawrence Salkoff’s lab) were amplified using primer pairs 4378/4379 and 4376/4377, respectively ( ).

    Cloning:

    Article Title: Melatonin Enhances Sleep via MT 1 -Driven Activation of Slo1 in Suprachiasmatic Nucleus Neurons
    Article Snippet: Normalized values were exported to Origin software (OriginLab, USA) for calculating the mean ± standard error (SE) and generating graphical representations. .. The plasmids encoding hemagglutinin (HA)-tagged mouse MT 1 ( wp2117 ) and FLAG-tagged mouse Slo1 ( wp2116 ) were constructed by cloning PCR-amplified products into the pIRES2–EGFP vector (Clontech, Mountain View, CA, USA). .. The Mtnr1a cDNA (NM_008639, MR224599, OriGene, Rockville, MD, USA) and Slo1 cDNA (MBr5/3, provided by Dr. Lawrence Salkoff’s lab) were amplified using primer pairs 4378/4379 and 4376/4377, respectively ( ).

    Polymerase Chain Reaction:

    Article Title: Melatonin Enhances Sleep via MT 1 -Driven Activation of Slo1 in Suprachiasmatic Nucleus Neurons
    Article Snippet: Normalized values were exported to Origin software (OriginLab, USA) for calculating the mean ± standard error (SE) and generating graphical representations. .. The plasmids encoding hemagglutinin (HA)-tagged mouse MT 1 ( wp2117 ) and FLAG-tagged mouse Slo1 ( wp2116 ) were constructed by cloning PCR-amplified products into the pIRES2–EGFP vector (Clontech, Mountain View, CA, USA). .. The Mtnr1a cDNA (NM_008639, MR224599, OriGene, Rockville, MD, USA) and Slo1 cDNA (MBr5/3, provided by Dr. Lawrence Salkoff’s lab) were amplified using primer pairs 4378/4379 and 4376/4377, respectively ( ).

    Article Title: Puromycin-sensitive aminopeptidase acts as an inhibitory auxiliary subunit of volume-regulated anion channels
    Article Snippet: Myc-Flag-tagged cDNAs of human SWELL1 (NM_019594), LRRC8B (NM_015350), LRRC8C (NM_032270), LRRC8E (NM_025061) in pCMV vector were obtained from OriGene (RC208632, RC205553, RC222603, RC209849). .. The coding sequences without a tag were PCR amplified and sub-cloned into pIRES2-EGFP vector (Clontech). .. The cDNA of human LRRC8D (NM_018130) in pCMV6-Entry vector was obtained from Amsbio(SC319580).

    Sequencing:

    Article Title: A humanized monoclonal antibody targeting an ectonucleotidase rescues cardiac metabolism and heart function after myocardial infarction
    Article Snippet: .. MGC Sequence-Verified human cDNAs of ENPP4, ENPP5, CD39, CD73 (Horizon Discovery), and ENPP3 (Cat# RDC2698, R&D Systems) were cloned into the pIRES2-EGFP vector (Clontech) for expression studies. ..

    Article Title: A humanized monoclonal antibody targeting an ectonucleotidase rescues cardiac metabolism and heart function after myocardial infarction.
    Article Snippet: MGC Sequence-Verified human cDNAs of ENPP4, ENPP5, CD39, CD73 (Horizon Discovery), and ENPP3 (Cat# RDC2698, R&D Systems) were cloned into the pIRES2-EGFP vector (Clontech) for expression studies. .. MGC Sequence-Verified human cDNAs of ENPP4, ENPP5, CD39, CD73 (Horizon Discovery), and ENPP3 (Cat# RDC2698, R&D Systems) were cloned into the pIRES2-EGFP vector (Clontech) for expression studies. ..

    Article Title: Enforced MYC expression directs a distinct transcriptional state during plasma cell differentiation
    Article Snippet: .. The MYC TAD MB deletion mutants, as well as the mutation of MYC 132-135 amino acid sequence, DCMW, to alanine residues, and the single substitution W135A were commercially synthesized with the t2A-BCL2 sequence included in the insert and cloned into the pIRES2-EGFP vector (Clontech). ..

    Article Title: Enforced MYC expression directs a distinct transcriptional state during plasma cell differentiation.
    Article Snippet: .. The MYC TAD MB deletion mutants, as well as the mutation of MYC 132-135 amino acid sequence, DCMW, to alanine residues, and the single substitution W135A were commercially synthesized with the t2A-BCL2 sequence included in the insert and cloned into the pIRES2-EGFP vector (Clontech). ..

    Clone Assay:

    Article Title: A humanized monoclonal antibody targeting an ectonucleotidase rescues cardiac metabolism and heart function after myocardial infarction
    Article Snippet: .. MGC Sequence-Verified human cDNAs of ENPP4, ENPP5, CD39, CD73 (Horizon Discovery), and ENPP3 (Cat# RDC2698, R&D Systems) were cloned into the pIRES2-EGFP vector (Clontech) for expression studies. ..

    Article Title: A humanized monoclonal antibody targeting an ectonucleotidase rescues cardiac metabolism and heart function after myocardial infarction.
    Article Snippet: MGC Sequence-Verified human cDNAs of ENPP4, ENPP5, CD39, CD73 (Horizon Discovery), and ENPP3 (Cat# RDC2698, R&D Systems) were cloned into the pIRES2-EGFP vector (Clontech) for expression studies. .. MGC Sequence-Verified human cDNAs of ENPP4, ENPP5, CD39, CD73 (Horizon Discovery), and ENPP3 (Cat# RDC2698, R&D Systems) were cloned into the pIRES2-EGFP vector (Clontech) for expression studies. ..

    Article Title: Enforced MYC expression directs a distinct transcriptional state during plasma cell differentiation
    Article Snippet: .. The MYC TAD MB deletion mutants, as well as the mutation of MYC 132-135 amino acid sequence, DCMW, to alanine residues, and the single substitution W135A were commercially synthesized with the t2A-BCL2 sequence included in the insert and cloned into the pIRES2-EGFP vector (Clontech). ..

    Article Title: Enforced MYC expression directs a distinct transcriptional state during plasma cell differentiation.
    Article Snippet: .. The MYC TAD MB deletion mutants, as well as the mutation of MYC 132-135 amino acid sequence, DCMW, to alanine residues, and the single substitution W135A were commercially synthesized with the t2A-BCL2 sequence included in the insert and cloned into the pIRES2-EGFP vector (Clontech). ..

    Expressing:

    Article Title: A humanized monoclonal antibody targeting an ectonucleotidase rescues cardiac metabolism and heart function after myocardial infarction
    Article Snippet: .. MGC Sequence-Verified human cDNAs of ENPP4, ENPP5, CD39, CD73 (Horizon Discovery), and ENPP3 (Cat# RDC2698, R&D Systems) were cloned into the pIRES2-EGFP vector (Clontech) for expression studies. ..

    Article Title: A humanized monoclonal antibody targeting an ectonucleotidase rescues cardiac metabolism and heart function after myocardial infarction.
    Article Snippet: MGC Sequence-Verified human cDNAs of ENPP4, ENPP5, CD39, CD73 (Horizon Discovery), and ENPP3 (Cat# RDC2698, R&D Systems) were cloned into the pIRES2-EGFP vector (Clontech) for expression studies. .. MGC Sequence-Verified human cDNAs of ENPP4, ENPP5, CD39, CD73 (Horizon Discovery), and ENPP3 (Cat# RDC2698, R&D Systems) were cloned into the pIRES2-EGFP vector (Clontech) for expression studies. ..

    Amplification:

    Article Title: Puromycin-sensitive aminopeptidase acts as an inhibitory auxiliary subunit of volume-regulated anion channels
    Article Snippet: Myc-Flag-tagged cDNAs of human SWELL1 (NM_019594), LRRC8B (NM_015350), LRRC8C (NM_032270), LRRC8E (NM_025061) in pCMV vector were obtained from OriGene (RC208632, RC205553, RC222603, RC209849). .. The coding sequences without a tag were PCR amplified and sub-cloned into pIRES2-EGFP vector (Clontech). .. The cDNA of human LRRC8D (NM_018130) in pCMV6-Entry vector was obtained from Amsbio(SC319580).

    Mutagenesis:

    Article Title: Enforced MYC expression directs a distinct transcriptional state during plasma cell differentiation
    Article Snippet: .. The MYC TAD MB deletion mutants, as well as the mutation of MYC 132-135 amino acid sequence, DCMW, to alanine residues, and the single substitution W135A were commercially synthesized with the t2A-BCL2 sequence included in the insert and cloned into the pIRES2-EGFP vector (Clontech). ..

    Article Title: Enforced MYC expression directs a distinct transcriptional state during plasma cell differentiation.
    Article Snippet: .. The MYC TAD MB deletion mutants, as well as the mutation of MYC 132-135 amino acid sequence, DCMW, to alanine residues, and the single substitution W135A were commercially synthesized with the t2A-BCL2 sequence included in the insert and cloned into the pIRES2-EGFP vector (Clontech). ..

    Synthesized:

    Article Title: Enforced MYC expression directs a distinct transcriptional state during plasma cell differentiation
    Article Snippet: .. The MYC TAD MB deletion mutants, as well as the mutation of MYC 132-135 amino acid sequence, DCMW, to alanine residues, and the single substitution W135A were commercially synthesized with the t2A-BCL2 sequence included in the insert and cloned into the pIRES2-EGFP vector (Clontech). ..

    Article Title: Enforced MYC expression directs a distinct transcriptional state during plasma cell differentiation.
    Article Snippet: .. The MYC TAD MB deletion mutants, as well as the mutation of MYC 132-135 amino acid sequence, DCMW, to alanine residues, and the single substitution W135A were commercially synthesized with the t2A-BCL2 sequence included in the insert and cloned into the pIRES2-EGFP vector (Clontech). ..



    Similar Products

    96
    TaKaRa pires2 egfp vector
    Pires2 Egfp Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pires2+egfp+vector/pIRES2-AcGFP1+Vector/bio_rxiv__2025__08__24__671966-144-12-14
    Average 96 stars, based on 1 article reviews
    pires2 egfp vector - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    93
    Addgene inc pires 2 egfp vector
    Pires 2 Egfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pires2+egfp+vector/pIRES2-EGFP+Mer+(Plasmid+%2314998)/bio_rxiv__2025__07__16__665058-25-17-21
    Average 93 stars, based on 1 article reviews
    pires 2 egfp vector - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    96
    TaKaRa mammalian expression vector pires2 egfp
    Mammalian Expression Vector Pires2 Egfp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pires2+egfp+vector/pIRES2-AcGFP1+Vector/pm40610563-374-13-17
    Average 96 stars, based on 1 article reviews
    mammalian expression vector pires2 egfp - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    95
    TaKaRa vector pires2 egfp
    Vector Pires2 Egfp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pires2+egfp+vector/pIRES2-DsRed2+Vector/pmc11819763-125-19-21
    Average 95 stars, based on 1 article reviews
    vector pires2 egfp - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    Image Search Results